Follicle stimulating hormone receptor in mesenchymal stem cells integrates effects of glycoprotein reproductive hormones
Identifieur interne : 002D48 ( Main/Exploration ); précédent : 002D47; suivant : 002D49Follicle stimulating hormone receptor in mesenchymal stem cells integrates effects of glycoprotein reproductive hormones
Auteurs : Irina L. Tourkova [États-Unis] ; Michelle R. Witt [États-Unis] ; La Li [États-Unis] ; Quitterie Larrouture [États-Unis] ; Li Liu [États-Unis] ; Jianhua Luo [États-Unis] ; Lisa J. Robinson [États-Unis] ; Harry C. Blair [États-Unis]Source :
- Annals of the New York Academy of Sciences [ 0077-8923 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Cellules stromales mésenchymateuses (), Cellules stromales mésenchymateuses (physiologie), Femelle, Glycoprotéines (pharmacologie), Gonadotrophine chorionique (pharmacologie), Grossesse, Hormone folliculostimulante (pharmacologie), Humains, Récepteur FSH (agonistes), Récepteur FSH (physiologie), Souris, Souris de souche-129, Souris knockout.
- MESH :
- agonistes : Récepteur FSH.
- pharmacologie : Glycoprotéines, Gonadotrophine chorionique, Hormone folliculostimulante.
- physiologie : Cellules stromales mésenchymateuses, Récepteur FSH.
- Animaux, Cellules cultivées, Cellules stromales mésenchymateuses, Femelle, Grossesse, Humains, Souris, Souris de souche-129, Souris knockout.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Chorionic Gonadotropin (pharmacology), Female, Follicle Stimulating Hormone (pharmacology), Glycoproteins (pharmacology), Humans, Mesenchymal Stromal Cells (drug effects), Mesenchymal Stromal Cells (physiology), Mice, Mice, 129 Strain, Mice, Knockout, Pregnancy, Receptors, FSH (agonists), Receptors, FSH (physiology).
- MESH :
- chemical , agonists : Receptors, FSH.
- chemical , pharmacology : Chorionic Gonadotropin, Follicle Stimulating Hormone, Glycoproteins.
- drug effects : Mesenchymal Stromal Cells.
- physiology : Mesenchymal Stromal Cells, Receptors, FSH.
- Animals, Cells, Cultured, Female, Humans, Mice, Mice, 129 Strain, Mice, Knockout, Pregnancy.
Abstract
Previously we reported that follicle stimulating hormone (FSH) affects bone degradation in human cells and in FSH-R null mice. Here we describe a FSH-R knockout bone formation phenotype. We used mesenchymal stem cells (MSCs), osteoblast precursors that express follicle stimulating hormone receptor (FSH-R), to determine whether FSH regulates bone formation. FSH stimulates MSC cell adhesion 1–3 h and proliferation at 24 h after addition. On the basis of phylogenetic and clinical precedents, we also examined effects of pregnant levels of human chorionic gonadotropin (hCG) on MSCs. We found effects similar to those of FSH, and RNAi knockdown of FSH-R abrogated both FSH and hCG effects on MSCs. In contrast to effects on MSCs, neither FSH nor hCG had significant effects on osteoblast maturation. Also in MSCs, short term treatment by FSH and hCG altered signaling pathways for proliferation, including Erk1/2 phosphorylation. Our results show augmentation of MSC proliferation by either FSH at menopausal levels or hCG at normal pregnant levels. We conclude that FSH-R participates in regulation of MSC precursor pools in response to either FSH or hCG, integrating the effects of these two glycoprotein hormones.
Url:
DOI: 10.1111/nyas.12502
PubMed: 25118101
PubMed Central: 4289445
Affiliations:
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Le document en format XML
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Tourkova</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Witt, Michelle R" sort="Witt, Michelle R" uniqKey="Witt M" first="Michelle R." last="Witt">Michelle R. Witt</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Li, La" sort="Li, La" uniqKey="Li L" first="La" last="Li">La Li</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Larrouture, Quitterie" sort="Larrouture, Quitterie" uniqKey="Larrouture Q" first="Quitterie" last="Larrouture">Quitterie Larrouture</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Luo, Jianhua" sort="Luo, Jianhua" uniqKey="Luo J" first="Jianhua" last="Luo">Jianhua Luo</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Robinson, Lisa J" sort="Robinson, Lisa J" uniqKey="Robinson L" first="Lisa J." last="Robinson">Lisa J. Robinson</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Blair, Harry C" sort="Blair, Harry C" uniqKey="Blair H" first="Harry C." last="Blair">Harry C. Blair</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Pathology, University of Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">Pittsburgh VA Medical Center Pittsburgh, Pennsylvania</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Pittsburgh VA Medical Center Pittsburgh</wicri:cityArea></affiliation></author></analytic><series><title level="j">Annals of the New York Academy of Sciences</title><idno type="ISSN">0077-8923</idno><idno type="eISSN">1749-6632</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chorionic Gonadotropin (pharmacology)</term><term>Female</term><term>Follicle Stimulating Hormone (pharmacology)</term><term>Glycoproteins (pharmacology)</term><term>Humans</term><term>Mesenchymal Stromal Cells (drug effects)</term><term>Mesenchymal Stromal Cells (physiology)</term><term>Mice</term><term>Mice, 129 Strain</term><term>Mice, Knockout</term><term>Pregnancy</term><term>Receptors, FSH (agonists)</term><term>Receptors, FSH (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cellules stromales mésenchymateuses ()</term><term>Cellules stromales mésenchymateuses (physiologie)</term><term>Femelle</term><term>Glycoprotéines (pharmacologie)</term><term>Gonadotrophine chorionique (pharmacologie)</term><term>Grossesse</term><term>Hormone folliculostimulante (pharmacologie)</term><term>Humains</term><term>Récepteur FSH (agonistes)</term><term>Récepteur FSH (physiologie)</term><term>Souris</term><term>Souris de souche-129</term><term>Souris knockout</term></keywords><keywords scheme="MESH" type="chemical" qualifier="agonists" xml:lang="en"><term>Receptors, FSH</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chorionic Gonadotropin</term><term>Follicle Stimulating Hormone</term><term>Glycoproteins</term></keywords><keywords scheme="MESH" qualifier="agonistes" xml:lang="fr"><term>Récepteur FSH</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Mesenchymal Stromal Cells</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Glycoprotéines</term><term>Gonadotrophine chorionique</term><term>Hormone folliculostimulante</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules stromales mésenchymateuses</term><term>Récepteur FSH</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mesenchymal Stromal Cells</term><term>Receptors, FSH</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, 129 Strain</term><term>Mice, Knockout</term><term>Pregnancy</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cellules stromales mésenchymateuses</term><term>Femelle</term><term>Grossesse</term><term>Humains</term><term>Souris</term><term>Souris de souche-129</term><term>Souris knockout</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Previously we reported that follicle stimulating hormone (FSH) affects bone degradation in human cells and in FSH-R null mice. Here we describe a FSH-R knockout bone formation phenotype. We used mesenchymal stem cells (MSCs), osteoblast precursors that express follicle stimulating hormone receptor (FSH-R), to determine whether FSH regulates bone formation. FSH stimulates MSC cell adhesion 1–3 h and proliferation at 24 h after addition. On the basis of phylogenetic and clinical precedents, we also examined effects of pregnant levels of human chorionic gonadotropin (hCG) on MSCs. We found effects similar to those of FSH, and RNAi knockdown of FSH-R abrogated both FSH and hCG effects on MSCs. In contrast to effects on MSCs, neither FSH nor hCG had significant effects on osteoblast maturation. Also in MSCs, short term treatment by FSH and hCG altered signaling pathways for proliferation, including Erk1/2 phosphorylation. Our results show augmentation of MSC proliferation by either FSH at menopausal levels or hCG at normal pregnant levels. We conclude that FSH-R participates in regulation of MSC precursor pools in response to either FSH or hCG, integrating the effects of these two glycoprotein hormones.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Tourkova, Irina L" sort="Tourkova, Irina L" uniqKey="Tourkova I" first="Irina L." last="Tourkova">Irina L. Tourkova</name></region><name sortKey="Blair, Harry C" sort="Blair, Harry C" uniqKey="Blair H" first="Harry C." last="Blair">Harry C. Blair</name><name sortKey="Blair, Harry C" sort="Blair, Harry C" uniqKey="Blair H" first="Harry C." last="Blair">Harry C. Blair</name><name sortKey="Larrouture, Quitterie" sort="Larrouture, Quitterie" uniqKey="Larrouture Q" first="Quitterie" last="Larrouture">Quitterie Larrouture</name><name sortKey="Li, La" sort="Li, La" uniqKey="Li L" first="La" last="Li">La Li</name><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name><name sortKey="Luo, Jianhua" sort="Luo, Jianhua" uniqKey="Luo J" first="Jianhua" last="Luo">Jianhua Luo</name><name sortKey="Robinson, Lisa J" sort="Robinson, Lisa J" uniqKey="Robinson L" first="Lisa J." last="Robinson">Lisa J. Robinson</name><name sortKey="Witt, Michelle R" sort="Witt, Michelle R" uniqKey="Witt M" first="Michelle R." last="Witt">Michelle R. Witt</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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